S1569

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S1569

Sigma

 

Stemline® Mesenchymal Stem Cell Expansion Medium

Without L-glutamine, liquid, sterile-filtered, cell culture tested

Synonym:MSC Medium

Description

General descriptionDeveloped to promote the optimal expansion of human mesenchymal stem cells (MSC) from bone marrow, Stemline Mesenchymal Stem Cell Expansion Medium demonstrates greater total nucleated cell (TNC) fold increases than other commercially available formulations. Additionally, functional trials clearly demonstrated Stemline's capacity to promote differentiation into adipocytes, chondrocytes, and osteocytes.

Produced in a GMP state-of-the-art facility with an available device master file (DMF), Stemline Hematopoietic Stem Cell Expansion Medium is clearly an excellent choice for clinical applications.

Stemline Mesenchymal Stem Cell Expansion Medium requires supplementation with antibiotics, cytokines, L-glutamine and fetal bovine serum, as appropriate to individual research protocols. Known to be extremely sensitive during initial isolation and growth ex vivo, MSC proliferation depends highly on the composition of fetal bovine serum (FBS) used to supplement their medium. Pre-screening with FBS is recommended, as the specific FBS components that effect MSC growth have not been fully identified.
ReconstitutionMedium must be supplemented with antibiotics, cytokines, 4 mM L-glutamine, and fetal bovine serum according to individual user protocols.
Legal InformationStemline is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

Properties

sterilitysterile-filtered
formliquid
qualityDrug/Device Master File available
suitabilitycell culture tested
shipped inwet ice
storage temp.2-8°C

Safety

Hazard CodesB
WGK Germany2

Related Products

ApplicationF2442, Fetal Bovine Serum
 G7513, L-Glutamine solution
 G8541, Ala-Gln

References

referenceMc.Niece, I. et al, Ex vivo expansion of cod blood mononuclear cell on mesenchymal stem cells Cytotherapy 6, 311-317, (2004) Abstract
 Moro, J., et al, Generation of gut-homing IgA-secreting B cells by intestinal dendritic cells Science 314, 1157, (2006) Abstract